ptre vector (TaKaRa)
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TaKaRa
ptre vector
Ptre Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptre+tight/pTRE-TIGHT+VECTOR/pmc12906178-156-13-15
Average 95 stars, based on 576 article reviews
Ptre Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptre+tight/pTRE-TIGHT+VECTOR/pmc12906178-156-13-15
Average 95 stars, based on 576 article reviews
ptre vector - by Bioz Stars,
2026-10
95/100 stars
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Clone Assay:Article Title: Methods and compositions for increasing protein expression and/or treating a haploinsufficiency disorder Article Snippet: .. All tRNA gene sequences were individually cloned into Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness Article Snippet: .. TetOff cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Article Title: Methods and compositions for increasing protein expression and/or treating a haploinsufficiency disorder Article Snippet: A nucleotide sequence encoding an ATA tRNA (tRNAATA) is depicted in SEQ ID NO: 1, a nucleotide sequence encoding an GTA tRNA (tRNAGTA) is depicted in SEQ ID NO: 2, and a nucleotide sequence encoding an AGA tRNA (tRNAAGA) is depicted in SEQ ID NO: 3, The SCN1A open reading frame was amplified by RT-PCR from human brain RNA (Clontech) using primers that contain a KpnI site in the forward primer and a NotI site in the reverse primer. .. SCN1A ORF was then cloned between the promoter and SV40 poly (A) site of Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness. Article Snippet: .. TetOff Oncogene cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Plasmid Preparation:Article Title: Nutrient availability-driven changes in extracellular matrix biochemical and mechanical properties regulate pancreatic cancer cell biology Article Snippet: .. Human ITGA2 ( hITGA2 ) cDNA containing plasmid was obtained from Sino Biological (HG13024-M) and subcloned into a modified version of Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness Article Snippet: .. TetOff cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness. Article Snippet: .. TetOff Oncogene cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II-DSIF-PAF1C complex. Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [27, 40]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [ , ]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Modification:Article Title: Nutrient availability-driven changes in extracellular matrix biochemical and mechanical properties regulate pancreatic cancer cell biology Article Snippet: .. Human ITGA2 ( hITGA2 ) cDNA containing plasmid was obtained from Sino Biological (HG13024-M) and subcloned into a modified version of FLAG-tag:Article Title: Nutrient availability-driven changes in extracellular matrix biochemical and mechanical properties regulate pancreatic cancer cell biology Article Snippet: .. Human ITGA2 ( hITGA2 ) cDNA containing plasmid was obtained from Sino Biological (HG13024-M) and subcloned into a modified version of Generated:Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness Article Snippet: .. TetOff cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Article Title: NELF and PAF1C complexes are core transcriptional machineries controlling colon cancer stemness. Article Snippet: .. TetOff Oncogene cell clones of the representative human colon cancer cells DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC: RRID:CVCL_1384) were generated using pTetOff (631017; TAKARA) and Amplification:Article Title: ERBB4 colocalizes with phosphorylated tau aggregates in multiple tauopathies. Article Snippet: .. To generate pTRE-Tight-hTau-FLAG and pEGFP-N1-hERBB4 plasmids, human tau (4R isoform) and ERBB4 (isoform b) cDNAs were amplified by PCR and subcloned into the Polymerase Chain Reaction:Article Title: ERBB4 colocalizes with phosphorylated tau aggregates in multiple tauopathies. Article Snippet: .. To generate pTRE-Tight-hTau-FLAG and pEGFP-N1-hERBB4 plasmids, human tau (4R isoform) and ERBB4 (isoform b) cDNAs were amplified by PCR and subcloned into the Sequencing:Article Title: Methods and compositions for increasing protein expression and/or treating a haploinsufficiency disorder Article Snippet: A nucleotide sequence encoding an ATA tRNA (tRNAATA) is depicted in SEQ ID NO: 1, a nucleotide sequence encoding an GTA tRNA (tRNAGTA) is depicted in SEQ ID NO: 2, and a nucleotide sequence encoding an AGA tRNA (tRNAAGA) is depicted in SEQ ID NO: 3, The SCN1A open reading frame was amplified by RT-PCR from human brain RNA (Clontech) using primers that contain a KpnI site in the forward primer and a NotI site in the reverse primer. .. SCN1A ORF was then cloned between the promoter and SV40 poly (A) site of Gene Expression:Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II-DSIF-PAF1C complex. Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [27, 40]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [ , ]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Concentration Assay:Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II-DSIF-PAF1C complex. Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [27, 40]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [ , ]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Expressing:Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II-DSIF-PAF1C complex. Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [27, 40]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [ , ]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Transfection:Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II-DSIF-PAF1C complex. Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [27, 40]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. Article Title: CDX1 and CDX2 suppress colon cancer stemness by inhibiting β-catenin-facilitated formation of Pol II–DSIF–PAF1C complex Article Snippet: TetOff cell clones were generated by transfecting the human colon cancer cell lines, DLD1 (CCL-221; ATCC) and LS174T (CL188; ATCC) with pTet-Off- (631017; TAKARA)-based plasmid vectors in accordance with the methodology in a previous study [ , ]. .. Gene expression was regulated by adding doxycycline (24390-14-5; TGI), a tetracycline analog, to the culture medium at a final concentration of 0.5 μg/mL. |
